IBA : NAPS

BODIPY^® fluorophores

BODIPY^® fluorophores span the complete visible spectrum and have spectral characteristics which are superior over commonly used dyes.
Advantages:

Higher quantum yields
Narrower emission bandwidths
Greater photostability
Insensitive to pH changes
Useful for DNA sequencing because the dye exhibits minimal effect on the mobility of the fragment during electrophoresis

Basic structure of the BODIPY fluorophore

BODIPY^® FL

The green fluorescent BODIPY^® FL fluorophore has several characteristics that make it potentially superior to fluorescein.
These include:

High extinction coefficient
High fluorescent quantum yield (often approaching 1.0, even in water)
Spectral properties that are insensitive to solvent polarity and pH
Narrow emission bandwidth, resulting in a higher peak intensity than fluorescein
Little or no spectral overlap with longer wavelength dyes such as tetramethyl-rhodamine and Texas Red, making it useful for multicolor applications
Greater photostability than fluorescein in some environments
Lack of ionic charge

The different BODIPY^® dyes are named according to their absorption and emission wave length or according to the dye they can substitute. The following BODIPY^® fluorophores are available:

BODIPY^®	absorption	emission	e [cm^-1M^-1]
493/503	500 nm	509 nm	79000
530/550	534 nm	551 nm	77000
558/568	559 nm	568 nm	97000
564/570-X	563 nm	569 nm	142000
576/589	575 nm	588 nm	83000
581/591-X	582 nm	591 nm	154000
630/650	625 nm	640 nm	101000
650/665	646 nm	660 nm	102000
FL (substitute for Fluorescein)	502 nm	510 nm	82000
R6G (substitute for Carboxyrhodamine 6G)	528 nm	547 nm	70000
TMR-X (substitute for TAMRA)	544 nm	570 nm	56000
TR-X (substitute for Texas-Red)	588 nm	616 nm	68000

Sold under license from Molecular Probes, Inc.

All BODIPY^® fluorophores are available in the following scales:

Order online

product	scale	amount	cat. no.	price/coupling
5'-BODIPY^®	0.05 µmol	5 nmol	5-0310-122	78.50 EUR
	0.20 µmol	25 nmol	5-0310-123	94.00 EUR
	1.00 µmol	100 nmol	5-0310-124	165.00 EUR
3'-BODIPY^®	0.05 µmol	5 nmol	5-0310-102	78.50 EUR
	0.20 µmol	25 nmol	5-0310-103	94.00 EUR
	1.00 µmol	100 nmol	5-0310-104	165.00 EUR
internal BODIPY^®	0.05 µmol	5 nmol	5-0310-112	100.00 EUR
	0.20 µmol	25 nmol	5-0310-113	115.00 EUR
	1.00 µmol	100 nmol	5-0310-114	165.00 EUR

Order information
see also "how to order"



		Reporter groups on oligonucleotides

Reporter groups on oligonucleotides

BODIPY® fluorophores

BODIPY® FL

BODIPY^® fluorophores

BODIPY^® FL