Licensed LNA™ amidites*

Providing unprecedented sensitivity and specificity

LNA ("Locked nucleic acid") is a novel proprietary nucleic acid analogue, which is easily incorporated in synthetic DNA oligonucleotides thereby providing unprecedented sensitivity, specificity and discriminatory power to a wide range of genomics products.


LNA probes have successfully been applied in
  • quantitative RT-PCR**-based expression profiling
  • SNP genotyping assays
  • expression microarrays
  • siRNA oligonucleotides for target validation
General properties of LNA oligonucletides
  • basepairs with DNA and RNA
  • exceptionally high thermal stability
  • improved discrimination
  • compatible with most enzymes
  • predictable melting behaviour
* Sold under license from Exiqon.
** The Polymerase Chain Reaction (PCR) is covered by patents owned by Roche Molecular Systems and F. Hoffmann-
La Roche Ltd.


LNA-Locked Nucleic Acid DNA RNA
LNA™ (Locked Nucleic Acid) is a bicyclic nucleic acid where a ribonucleoside is linked between the 2'-oxygen and the 4-carbon atoms with a methylene unit.

Oligonucleotides containing LNA nucleotides exhibit unprecedented thermal stabilities towards complementary DNA and RNA as seen in table 1.
Type Duplexes Tm (°C) Δ Tm (°C)/mod.

DNA:DNA

 5'-tga gct ggt ggg-3'
3'-act cga cca ccc-5'		 35.0 -
LNA:DNA 5'-tGa GcT gGt Ggg-3'
3'-act cga cca ccc-5'		 52.5 +3.5
DNA:RNA 5'-gtg ata tgc-3'
3'-cac uau acg-5'		 28 -
LNA:RNA 5'-gTg aTa Tgc-3'
3'-cac uau acg-5'		 50 +7.3
Table 1: LNA monomers in upper case and bold; DNA/RNA monomers in lower case.


Additionally, LNA show excellent mismatch discrimination. In fact, the high binding affinity of LNA oligonucleotides allows for the use of short probes in e.g. SNP Genotyping, see table 2.
Tm 's of duplexes Perfect match
3'-acgaccac		 Single mismatch
3'-acggccac		 Δ Tm
5'-TGCTGGTG 71 °C 45 °C 26 °C
5'-tgctggtg 35 °C 25 °C 10 °C
Table 2: LNA monomers in upper case; DNA monomers in lower case.


References
Petersen M, Wengel J, 2003: TRENDS in Biotechnol 21(2):74-81.
Mouritzen P, Toftgaard Nielsen A, Pfundheller HM, Choleva Y, Kongsbak L, Moller S, 2003: Expert Rev Mol Diagn 3(1):27-38.
Grünweller A, Wyszko E, Bieber B, Jahnel R, Erdmann VA, Kurreck J, 2003: Nucl Acids Res 31(12):3185-3193.
Kurreck J, Wyszko E, Gillen C, Erdmann VA, 2002: Nucl Acids Res 30(9):1911-1918.
Order information
see also "how to order"

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description amount
cat. no.
 price
LNA coupling 0.05 µmol scale 5-0415-002 20,00 EUR
LNA coupling 0.2 µmol scale 5-0415-003 30,00 EUR
LNA coupling 1.0 µmol scale 5-0415-004 40,00 EUR




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