Magnet Assisted Lipofection
MA Lipofection Enhancer
Transfection with common lipidic or polycationic reagents can be enhanced by magnetic assistance ("Magnet Assisted Lipofection"). In this case, the nucleic acid to be transfected has to be combined with MA Lipofection Enhancer in the presence of a common transfection reagent. The formulation of MA Lipofection Enhancer, which contains magnetic nanoparticles (MagTag™), has been optimized for use with IBA's new lipofection reagent IBAfect. The MA Lipofection Enhancer can be used for adherent cells; for suspension cells see MATra-S Immobilizer.
MATra-S Immobilizer
Suspension cells have to be made adherent first by incubating them with the reagent MATra-S Immobilizer which contains magnetic nanoparticles (MagTag™). Then MATra-A Reagent (or MA Lipofection Enhancer with IBAfect) loaded with the nucleic acid can be applied and MATra (or Magnet Assisted Lipofection) can be performed.
IBAfect
IBAfect is a pentacationic transfection reagent based on DNA/RNA/lipid-complex technology. The specifically designed molecular structure of the cationic lipid ensures easy entry of DNA/RNA into cells by condensing DNA/RNA to compact structures (DNA/RNA/lipid-complex) efficiently entering the cell by endocytosis. The DNA/RNA/lipid-complexes act like "proton sponges" which causes lysis of the endosome. Nucleic acids are released simultaneously from the complex by "Progressive Proton-assisted Lipid Layer Disintegration" (P.P.L.L.D.). IBAfect is provided as a ready-to-use solution. It shows no serum inhibition, which makes it a reagent of choice for transfecting sensitive cell lines. IBAfect is recommended to be combined with IBA's MA Lipofection Enhancer.
We recommend trying MATra-A Reagent and the MA Lipofection Enhancer (with IBAfect) in parallel to find the optimal solution for your cells of interest!
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