StarGate^® - Combinatorial Cloning in one Tube

Evaluate the best protein-tags and hosts for the expression of your gene of interest very fast and easy

StarGate Overview^®

Click here for

• transfer reaction details
  
  

StarGate® procedure Step 1: Entry reaction In a first step, the gene of interest (GOI) is extended at both ends with the combinatorial and StarCombinase sites by PCR (orange and red; primers). Then, the PCR product is inserted into an Entry Vector by a simple one-tube reaction using a specific StarCombinase. Optional intermediate step: Fusion Cloning Via an optional intermediate step between entry and transfer reaction you can construct artificial operons for multiple gene expression in bacterial or mammalian cells or generate fusion proteins. Step 2: Transfer reaction After sequence confirmation, the resulting Donor Vector is the source for the highly parallel subcloning of the GOI by a second simple one tube reaction into a multitude of Acceptor Vectors, each providing a different genetic surrounding like host specific promoters and different purification tags. The resulting Destination Vectors are then transformed into the corresponding host cells for further experiments. Acceptor Vectors are listed in the Acceptor Vector Overview Table.

We offer an easy-to-use software, the StarPrimer D´Signer 2.0, to facilitate the design of primers for the ENTRY Vectors both for StarGate Standard Cloning and for StarGate Mutagenesis Cloning. The software guides you step-by-step through the whole PCR procedure.

• Please download StarGate manual for detailed information