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Vectors for mammalian expression |
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| pEXPR-IBA vectors are designed for high-level expression and purification of recombinant Strep-tag® and/or 6xHistidine-tag fusion proteins in mammalian cells. The vectors provide the same cloning strategy and are, thus, compatible with the corresponding bacterial pASK-IBA plasmids (see vectors for E. coli). As a consequence a PCR fragment can be cloned into pASK-IBA and its pEXPR-IBA equivalent in parallel (e.g. pASK-IBA3 and pEXPR-IBA3). The human cytomegalovirus (CMV) immediate early promoter provides strong expression in a wide range of mammalian cells. To prolong expression in transfected cells, the vector will replicate in cell lines that are latently infected with SV40 large T-antigen (e.g. COS7). In addition, the Neomycin resistance gene allows direct selection of stable cell lines. |
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Order information see also "how to order" |
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