Vector overview

E. coli

pASK-IBA/pPR-IBA vectors (ampicillin resistance) Note concerning "plus" versions: The cytoplasmic pASK-IBA vectors are now offered in an improved version. They contain a translation initiation site with higher efficiency increasing primary protein yield. These plasmids are denoted additionally with "plus" (e.g. cat. no. 2-1404-000 "pASK-IBA5plus"). They contain the same multiple cloning site* as the original plasmid (e.g. "pASK-IBA5") and differ only in the translation initiation site. (*Exception: pASK-IBA43plus contains a NheI restriction site between the ATG start codon and the N­terminal 6xHis tag, which is not included in pASK-IBA43.) The old versions are discontinued and are only available on special request. For individual maps please refer to the data sheets.

pASK-IBA vectors (chloramphenicol resistance) For individual maps please refer to the data sheets.

Pre-cut pASK-IBA/pPR-IBA vectors

Sequencing primers

PCR cloning with pASK-IBA, pPR-IBA and pEXPR-IBA vectors (pdf, 130 KB)

Multiple cloning sites, maps and sequences

Mammalia

Yeast

All vectors at a glance

Plasmid	Strep-tag	6xHistidine -tag	HA epitope-tag	Secretion	Removal of the tag	protease	resistance	cat. no.	order & prices
E. coli vectors with tet promoter for periplasmic expression
pASK-IBA2	C-terminal	-	-	Yes	No	not applicable	Amp	2-1301-000
pASK-IBA4	N-terminal	-	-	Yes	No	not applicable	Amp	2-1303-000
pASK-IBA6	N-terminal	-	-	Yes	Yes	factor Xa	Amp	2-1305-000
pASK-IBA12	N-terminal	-	-	Yes	Yes	thrombin	Amp	2-1311-000
pASK-IBA14	N-terminal	-	-	Yes	Yes	enterokinase	Amp	2-1313-000
pASK-IBA16	N-terminal	-	-	Yes	Yes	TEV protease	Amp	2-1315-000
pASK-IBA32	-	C-terminal	-	Yes	No	not applicable	Amp	2-1332-000
pASK-IBA44	N-terminal	C-terminal	-	Yes	No	not applicable	Amp	2-1344-000
E. coli vectors with tet promoter for cytoplasmic expression
pASK-IBA3plus	C-terminal	-	-	No	No	not applicable	Amp	2-1402-000
pASK-IBA5plus	N-terminal	-	-	No	No	not applicable	Amp	2-1404-000
pASK-IBA7plus	N-terminal	-	-	No	Yes	factor Xa	Amp	2-1406-000
pASK-IBA13plus	N-terminal	-	-	No	Yes	thrombin	Amp	2-1412-000
pASK-IBA15plus	N-terminal	-	-	No	Yes	enterokinase	Amp	2-1414-000
pASK-IBA17plus	N-terminal	-	-	No	Yes	TEV protease	Amp	2-1416-000
pASK-IBA33plus	-	C-terminal	-	No	No	not applicable	Amp	2-1433-000
pASK-IBA35plus	-	N-terminal	-	No	No	not applicable	Amp	2-1435-000
pASK-IBA37plus	-	N-terminal	-	No	Yes	factor Xa	Amp	2-1437-000
pASK-IBA43plus	C-terminal	N-terminal	-	No	No	not applicable	Amp	2-1443-000
pASK-IBA45plus	N-terminal	C-terminal	-	No	No	not applicable	Amp	2-1445-000
pASK-IBA63a-plus	C-terminal	-	-	No	No	not applicable	Amp	2-1463-100
pASK-IBA63b-plus	C-terminal	-	-	No	No	not applicable	Amp	2-1463-200
pASK-IBA63c-plus	C-terminal	-	-	No	No	not applicable	Amp	2-1463-300
pASK-IBA65b-plus	N-terminal	-	-	No	No	not applicable	Amp	2-1465-200
E. coli vectors with tet promoter and Chloramphenicol resistance (CAT)
pASK-IBA2C	C-terminal	-	-	Yes	No	not applicable	CAT	2-1321-000
pASK-IBA3C	C-terminal	-	-	No	No	not applicable	CAT	2-1322-000
pASK-IBA4C	N-terminal	-	-	Yes	No	not applicable	CAT	2-1323-000
pASK-IBA5C	N-terminal	-	-	No	No	not applicable	CAT	2-1324-000
pASK-IBA6C	N-terminal	-	-	Yes	Yes	factor Xa	CAT	2-1325-000
pASK-IBA7C	N-terminal	-	-	No	Yes	factor Xa	CAT	2-1326-000
E. coli vectors with T7 promoter
pPR-IBA1	C-terminal	-	-	No	No	not applicable	Amp	2-1390-000
pPR-IBA2	N-terminal	-	-	No	No	not applicable	Amp	2-1391-000
Mammalian vectors with human CMV promoter
pEXPR-IBA3	C-terminal	-	-	No	No	not applicable	Amp/Neo	2-1903-000
pEXPR-IBA5	N-terminal	-	-	No	No	not applicable	Amp/Neo	2-1905-000
pEXPR-IBA7	N-terminal	-	-	No	Yes	factor Xa	Amp/Neo	2-1907-000
pEXPR-IBA13	N-terminal	-	-	No	Yes	thrombin	Amp/Neo	2-1913-000
pEXPR-IBA15	N-terminal	-	-	No	Yes	enterokinase	Amp/Neo	2-1915-000
pEXPR-IBA17	N-terminal	-	-	No	Yes	TEV protease	Amp/Neo	2-1917-000
pEXPR-IBA42	C-terminal	N-terminal	-	Yes	No	not applicable	Amp/Neo	2-1942-000
pEXPR-IBA43	C-terminal	N-terminal	-	No	No	not applicable	Amp/Neo	2-1943-000
pEXPR-IBA44	N-terminal	C-terminal	-	Yes	No	not applicable	Amp/Neo	2-1944-000
pEXPR-IBA45	N-terminal	C-terminal	-	No	No	not applicable	Amp/Neo	2-1945-000
Yeast vectors with ADH2 promoter
pKS1-ST	N-terminal	-	N-terminal	No	No	not applicable	Amp/G418	2-3801-000
pKS2-ST	N-terminal	-	N-terminal	Yes	No	not applicable	Amp/G418	2-3802-000

NEW! pASK-IBA/pPR-IBA vectors pre-cut with BsaI

For our special cloning strategy using BsaI see
http://www.iba-go.com/prottools/index.php?prot_p_clsy.html
For individual maps please refer to the data sheets.

Please inquire for pre-cut vectors at info@iba-go.com.

Sequencing primers

Product	amount	cat. no.	order & prices
Forward sequencing primer for pASK-IBA vectors	1nmol, 10 pmol/µl HPLC purified	5-0000-101
Reverse sequencing primer for pASK-IBA vectors	1nmol, 10 pmol/µl HPLC purified	5-0000-102
Forward and reverse sequencing primers for pASK-IBA vectors	1 nmol each, 10 pmol/µl HPLC purified	5-0000-104
Forward sequencing primer for pPR-IBA vectors	1nmol, 10 pmol/µl HPLC purified	5-0000-111
Reverse sequencing primer for pPR-IBA vectors	1nmol, 10 pmol/µl HPLC purified	5-0000-112
Forward and reverse sequencing primer for pPR-IBA vectors	1 nmol each, 10 pmol/µl HPLC purified	5-0000-114
Forward sequencing primer for pEXPR-IBA vectors	1nmol, 10 pmol/µl HPLC purified	5-0000-121
Reverse sequencing primer for pEXPR-IBA vectors	1nmol, 10 pmol/µl HPLC purified	5-0000-122
Forward and reverse sequencing primers for pEXPR-IBA vectors	1 nmol each, 10 pmol/µl HPLC purified	5-0000-124

Order information
see also "how to order"