Antibody columns

This new antibody-based purification column with StrepMAB-Classic immobilized to MacroPrep^® provides an alternative for purification  of Strep-tag^® proteins. Using this column as second step following purification via Strep-Tactin^® protein purity can be increased to over 99% while only one tag – the Strep-tag^® – is used.

In protein:protein interaction analysis e.g. this column is used in the so-called One-TAP system enabling a second, completely independent purification step with respect to resin (MacroPrep^® vs Superflow^®) and affinity receptor (mAB vs Strep-Tactin^®).

Strep-tag II Peptide

For elution of Strep-tag proteins from StrepMAB-Classic columns

Gravity flow StrepMAB-Immo MacroPrep^® Column

Stable immobilization of proteins on columns

StrepMAB-Immo, a murine monoclonal antibody, binds SerAla-One-STrEP-tag (and SerAla- Strep-tag II) fusion proteins nearly irreversibly. Therefore StrepMAB-Immo columns are designed for the stable and oriented immobilization of proteins. Analyzing e.g. the interaction of proteins the column can be a helpful tool, since the interactome can be immobilized via a Strep-tagged bait protein. The prey proteins may then be sequentially eluted from the bait using adequate buffers. The bait proteins are presumed to stay bound under a wide
range of buffer conditions.

Note: Gravity flow StrepMAB-Immo MacroPrep Columns are not recommended for the purification of One-STrEP-tag or Strep-tag^®II fusion proteins because elution is not possible under physiological conditions

Columns used e.g. for sequential elution of prey proteins in protein:protein interaction studies