for the fast detection of Strep-tag II proteins in Western blots
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- Chromogenic Detection (for chemiluminescence detection please click here)
For chromogenic detection of Strep-tag proteins on Western blots or ELISA we offer
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Specifications of Strep-Tactin AP conjugate
| Alkaline Phosphatase |
Calf intestine |
| Detection |
N- or C-terminal or internal Strep-tag II |
| Sensitivity in dot blots |
> 1 ng/dot |
| Sensitivity in Western blots |
> 2 ng/band |
| Cross reactivity |
Reacts with Biotin Carboxyl Carrier Protein (BCCP, 22.5 kDa) in E. coli extracts. Generally, biotinylated proteins can be sufficiently masked by a 10 minute preincubation with 2 µg/ml Biotin Blocking Buffer. |
| Form |
Suspended in 0.01 M phospate buffered saline pH 7.4 containing BSA and 0.01 % Thimerosal as preservative. Can be diluted 1:4,000 for detection of Strep-tag II fusion proteins after Western blotting. (Please note that for ELISA we recommend to use the Strep-Tactin HRP conjugate) |
| Stability |
1 year |
| Storage |
4 °C |
| Shipment |
RT |
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The Strep-tag AP Detection Kit
Strep-tag Protein Ladder is provided as positive control protein within the AP Detection Kit and can also be purchased separately
(#2-1011-100 sufficient for 100 Applications) |
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Kit components (sufficient for 1000 cm2 membrane)
| Component |
Description |
Amount |
| Control protein |
Recombinant Strep-tag II fusion protein |
24 µg |
| Buffer SI |
Blocking and incubation buffer 10x |
50 ml |
| Buffer W |
Washing buffer 20x |
60 ml |
| Strep-Tactin AP conjugate |
at least 2 mol AP / mol Strep-Tactin |
0.125 ml |
| Buffer ER |
AP reaction buffer 10x |
25 ml |
| AP substrate |
BCIP* (5-bromo-4-chloro-3-indolylphosphate p-toluidine salt) |
1 ml |
| Chromogen |
NBT* (nitroblue tetrazolium chloride) |
1 ml |
*Store BCIP and NBT solutions immediately at - 20 °C upon arrival.
All other solutions can be stored at 4-8 °C until use. Shipment at RT.
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Need buying or technical advice?
Contact a protein expert!
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- +49(0)551 50672-0
- Online
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Order information
see also "how to order"
| product |
amount |
cat. no. |
order & prices |
| Strep-tag AP Detection Kit |
1 kit |
2-1503-000 |
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| Strep-Tactin AP conjugate |
0.5 ml |
2-1503-001 |
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| Strep-tag Protein Ladder (lyophilized) |
240 µg; 100 applications |
2-1011-100 |
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