Detection via 6xHistidine-tag
Primary antibody against 6xHistidine-tag: Mouse anti 6xHistidine mAb, lyophilized
| Description |
Anti 6xHistidine-tag monoclonal antibody; purified by affinity chromatography |
| Subclass |
IgG1 |
| Specificity |
6xHistidine-tag; the antibody was produced in mouse using 6xHistidine-tag linked to PAX6 transcription factor as immunogen |
| Detection |
N- or C-terminal or internal 6xHistidine-tag |
| Form |
Lyophilized from NH4HCO3, contains 10 mg sucrose per mg protein |
| Reconstitution |
Reconstitute in phosphate buffered saline (PBS) at a concentration of 0.2 – 0.1 mg/ml (i. e. dissolve with
0.5 – 1.0 ml PBS, respectively). Aliquot and store at 20 °C. |
| Dilutions |
Recommended dilution for detection of 6xHistidine-tag fusion proteins in Western blots (chromogenic detection):
- 100-200 ng/ml diluted in PBS containing 0.2% BSA
- Secondary antibody: rabbit anti-mouse pAb, HRP conjugated; Cat. No. 2 1591-001
|
| Amount |
100 µg; sufficient to develop 50-100 Western Blots using
10 ml incubation volume each |
| Stability |
12 months after shipping |
| Storage |
-20 °C |
| Shipment |
RT |
|
