|
Strep-tag fusion protein |
Strep-tag application (for references click here) |
Large proteins
Oat phytochrome A,
124 kDa |
VH domain of the lysozyme-binding D1.3 Fv fragment (mouse hybridoma) |
Purification of the intact Fv fragment;
Detection of the Fv fragment via ELISA and Western blotting |
| VL domain of the D1.3 Fv fragment (mouse hybridoma) |
Purification of the intact Fv fragment;
Detection of the Fv fragment via ELISA and Western blotting;
Capture of the Fv:antigen complex |
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Azurin (P. aeruginosa) |
Purification of the functional metalloprotein |
| Cytochrome b562 (E. coli) |
Purification of the functional heme protein |
| Cystatin (chicken) |
Purification of the functional protein |
| Retinol-binding protein (pig) |
Purification of the functional protein |
| Bilin-binding protein and mutants (Pieris brassicae) |
Purification of the functional protein (47);
ELISA applications (47) |
| Fragment of the growth-promoting activity receptor A (chicken) |
Purification of the functional protein fragment |
Reference:
Murphy J T & Lagarias J C, 1997: Photochem. Photobiol., 65, 750-758. Purification and characterization of recombinant affinity peptide-tagged oat phytochrome A. |
Fragment of the ciliary neurotrophic factor receptor (chicken) |
Purification of the functional protein fragment |
| VH domain of the cytochrome C oxidase-binding 7E2 Fv fragment (mouse hybridoma) |
Purification of the intact Fv fragment (44);
Capture of the intact cytochrome C oxidase (4 subunits) (19);
Detection of the Fv fragment via immunoelectron microscopy (53) and immunofluorescence microscopy (56) |
Multimeric membrane protein complexes
P. denitrificans cytochrome c oxidase |
VH domains of the ubiquinol:cytochrome Coxidoreductase-binding 7D3 and 2D6 Fv fragments (mouse hybridomas) |
Purification of the intact Fv fragment (53);
Capture of the intact ubiquinol:cytochrome C oxidoreductase (3 subunits) (19);
Detection of the Fv fragment via immunoelectron microscopy (53, 54) and immunofluorescence microscopy (56) |
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VH domain of the photosystem I - binding 1E7 Fv fragment (mouse hybridoma) |
Purification of the intact Fv fragment (55);
Capture of the intact photosystem I (3 subunits) (55)
Detection of the Fv fragment via Western blotting (55) and immunoelectron microscopy (55) |
| Phytochrome A (oat) |
Purification of the functional apo-protein (expressed in yeast) (17) |
| Alkaline phosphatase (E. coli) |
Purification of the active metalloenzyme (32) |
| Green fluorescent protein (A. victoria) |
Purification of the functional protein (2);
Detection in Western blot |
Reference:
Kleymann G, Ostermeier C, Ludwig B, Skerra A & Michel H, 1995: Bio/ Technology 13, 155-160. Engineered Fv fragments as a tool for the one-step purification of integral multisubunit membrane protein complexes. |
Subunit B of H. pylori urease |
Copurification of expressed A and B subunits (4);
Detection in Western blot (Subunit B) |
| Curli assembly factor CsgG (E. coli outer membrane) |
Purification of the native outer membrane protein (8) |
| Phytochrome Cph1 (Cyanobacteria) |
Purification of the active, light-regulated histidine kinase |
|
Glutathione S-transferase and fusion proteins |
Purification of the dimeric fusion enzyme (33);
Detection in ELISA (33) |
Metalloenzymes
E. coli alkaline phosphatase |
VH domain of the cytochrome C oxidase-binding 7E2C50S Fv fragment (mouse hybridoma) |
Purification of the intact Fv fragment (38);
Capture of the cytochrome c oxidase subunits I and II (38) |
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Hydrogenase precursor (E. coli) |
Purification of the active precursor with N-terminal Strep-tag II (25) |
| PdxA and PdxJ (E. coli) |
Purification of the active enzymes for vitamin B6 synthesis (23) |
| Hyaluronate synthase (group A streptococci) |
Purification of the active enzyme, probably in membrane-anchored form (1) |
| SP1 and SP3 transcription factors |
Purification of the soluble proteins (expressed in SF9 cells) (1) |
| Ligase I, III, IV, and XRCC4 (human) |
Purification of the active enzymes (expressed in HeLa cells) (1) |
Reference:
Hengsakul M, Cass AEG, 1997: J. Mol. Biol. 266: 621-632.
Alkaline phosphatase-Strep-tag fusion protein binding to streptavidin: Resonant mirror studies. |
T7 RNA polymerase (E. coli) |
Purification of the active enzyme (1) |
| CPP32 (human) |
Purification of the active cysteine protease (1) |
| BetP (C. glutamicum) |
Purification of this integral membrane protein for glycine betaine uptake exhibiting the same transport characteristics compared to the wild-type protein (39) |
| NADH dehydrogenase fragment of the so called complex I (E. coli) |
Purification of the correctly assembled complex composed of 3 subunits, one containing the Strep-tag II (40) |
|
BHLHzip domain of c-Myc (human) |
Purification of the correctly folded protein domain binding to its cognate ligand "Max" (21) |
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Tissue transglutaminase, the major autoantigen of gluten-sensitive enteropathy (human) |
Purification of the active enzyme which had been expressed in the human embryonic kidney cell line 293-EBNA (14) |
