Heterogeneous FasL-Strep Apoptosis Assay
In the heterogeneous apoptosis assay format, the FasL-Strep molecule is immobilized on Strep-Tactin® (the cognitive receptor of Strep-tag®) coated microplates. After addition of the cells to be investigated, caspase 3/7 activity is determined for measuring extent of apoptosis.
The FasL-Strep apoptosis assay described above is robust and reproducible due to the high stability of the FasL-Strep reagent. The test is easy to perform since common reagents are applied for read out. These properties identify this assay as an ideal basis for HTS assays for screening anti-apoptotic molecules.
The assay is available for human or murine FasL-Strep on Strep-Tactin® coated microplates.
Components of the Heterogeneous FasL-Strep Apoptosis Assay are:
2.5 µg FasL-Strep (human or murine, respectively) and 1 Strep-Tactin® coated microplate, 96-well.
Homogeneous FasL-Strep Apoptosis Assay
The homogeneous FasL-Strep Apoptosis Assay can be performed in the presence of the anti-Strep-tag® antibody "StrepMAB-Immo" which increases apoptotic activity of FasL-Strep. The homogeneous FasL-Strep assay enables the quantitative determination of anti-apoptotic agents as shown by Fas-Fc competition. The measurement of apoptosis in the respective cells is achieved by determination of caspase 3/7 activity.
The assay is available with human or murine FasL-Strep and includes StrepMAB-Immo.
Components of the Homogeneous FasL-Strep Apoptosis Assay are:
2.5 µg FasL-Strep (human or murine, respectively) and 100 µg StrepMAB-Immo (Strep-tag antibody which increases the inducing effect of FasL-Strep).
Single Products
Please note that you can order FasL-Strep and the other components of the FasL-Strep assays also as single products. Please see order table for an overview.
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