Features
- One-step purification from crude lysates
- High binding affinity and high capacity
- Purification under native or denaturing conditions is possible
- Pre-charged, ready-to-use matrices for any scale of purification
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| Tighter binding of nickel ions with NTA |
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Comparison of the interactions of different metal-chelating resins with nickel ions.
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| Tighter binding of the 6xHistidine-tag |
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| The capture of 6xHistidine-tag proteins by metal-chelate affinity matrices relies on two interactions. Both are important for optimal performance. If interaction A is weak, there is no binding of the 6xHistidine-tag protein. If interaction A is strong, but interaction B weak, protein is lost as protein-metal complexes during wash steps. When NTA ligand and nickel are used to bind 6xHistidine-tag molecules, both interactions are stronger, providing advantages over systems that rely on other ligands or metals. |
