Highly pressure resistant
Ni-NTA Superflow cartridges H-PR (1 ml) for direct use with workstations (no adapters needed for 10-32 connections)
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Ni-NTA Superflow combines superior mechanical stability and outstanding flow characteristics with high dynamic capacity. This resin allows purification of 6xHistidine-tag proteins under gravity flow as well as high flow rates and pressures for efficient FPLC applications. Pre-packed columns (1 and 5 ml) for gravity flow as well as pre-packed H-PR cartridges (1 and 5 ml) for low pressure chromatography by means of syringes, peristaltic pump systems or FPLC/HPLC workstations are available. Cartridges can be connected in series to enlarge capacity. Adapters for use of the H-PR cartridges with syringes, peristaltic pumps or HPLC/FPLC workstations are described here.
Please note that no adapters are needed for use of the cartridges with Äkta or HPLC with 10-32 connections.
| Specifications Ni-NTA Superflow |
| Binding capacity: |
Up to 50 mg/ml (up to 2500 nmol @ ~20 kDa) |
| Support: |
Superflow |
| Bead structure: |
Highly cross-linked, 6% agarose |
| Bead size: |
60-160 µm |
| Form: |
50% suspension in 30% ethanol, precharged with Ni2+ |
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