IBA Gene TAGnologies

Cloning/gene expression

• Expression systems
  • Tet /Anhydrotetracycline
  • T7
  • Remarks
    
    
• Vector description
  • Overview
  • Sequencing primers
  • Maps E. coli vectors
  • Maps mammalian vectors
  • Multiple Cloning Sites
  • Sequences
    
    
• Cloning system
  
  
• Primer D'Signer
  

Magnet Assisted Transfection

• MATra Principle/Advantages
  
  
• Methods
  • Scheme
  • Easy protocol
  • Nucleic Acid / Virus
  • FAQ
    
    
• Reagents
  • MA Transfection
  • MA Lipofection
  • Low vector dosis
  • Assay Formats
    
    
• Transfected Cells
  
  
• Magnet Plates
  
  
• Examples
  • Mouse Fibrosarcoma
  • FaDu Carcinoma
  • Hepatocellular Carcinoma
  • Neurons
  • Primary Hepatocytes
  • VSa 13
    
    
• siRNA
  • Principle
  • Examples/Applications
  • Order Information
    
    
• Informations
  • Order information
  • Manuals
  • Trouble Shooting

Nucleic acid specialties

• General info
  
  
• Purification
  • Molecular Biology
  • HPLC
  • Double HPLC
  • RNA HPLC
  • DS (desalting)
  • Cell culture
  • PAGE
  • Reverse phase C18
  • Documentation
    
    
• D N A
  • Standard
  • Long range
  • Large scale
    
    
• Hybrids
  
  
• R N A
  • Double strand
  • Standard
  • Modified
  • Transcription starters
    
    
• Antisense
  
  
• Modifications
  • 5'-modified
  • 3'-modified
  • internal
    • Adenosine
    • Cytidine
    • Guanosine
    • Thymidine
    • Others
      
      
• Labels
  • Fluorescence
    • Dyes
    • Real-time PCR
    • FRET
  • Biotin/Dig
    • Biotin
    • Iminobiotin
    • Oligo(dT)₂₀
    • Dig
      
      
• NTPs
  • Deoxy-NTPs
  • Ribo-NTPs
  • alpha-thio-NTPs
    
    
• References
  

IBA Protein TAGnologies

Purification via tags/products

• Strep-tag
  • Technology
    • The Strep-tag story
    • Principle & properties
      
  • Cloning/gene expression
  • Examples
  • Purification
    • Introduction
    • Applications
    • Strep-tag Starter Kits
    • Resin Specifications
    • Column formats
    • Spin columns
    • Evaluation Sets
    • Strep-Tactin Sepharose
    • Strep-Tactin Superflow
    • Strep-Tactin MacroPrep
    • Strep-Tactin POROS
    • MagStrep
    • Buffers & Reagents
    • Tips & Tricks
  • Detection
    • Introduction
    • HRP detection
    • AP detection
    • Antibodies
    • Protein ladder
  • Assay
  • Latest press/references
    
    
• 6xHistidine-tag
  • Introduction
  • Strep/His Starter Kit
  • Ni-NTA system
  • Ni-NTA Sepharose
  • Ni-NTA Superflow
    
    
• Double-tag
  
  
• Protein interaction
  
  
• High-throughput

Purification via tags/services

• High-throughput
  
  
• Expression & Purification
  • Cloning
  • Gene synthesis
  • Expr. & Purification
    • E. coli
    • S. cerevisiae
    • Insect cells
  • Proteolytic trimming
  • Requirements
  • General procedure

Protein analysis

• N-terminal sequencing
  
  
• Mass spectrometry
  • MALDI/TOF
  • ESI
    
    
• 2D Gels & more
  
  

IBA Cell TAGnologies

CD8+-T-cell staining & isolation

• Technology
  • Summary
  • Background
  • The Steptamer approach
  • Magnetic Beads
  • Experimental data
  • T-cell overview
    
    
• Products
  
  
• Custom service

Magnet Assisted Transfection

Monoclonal antibody service

GMP & more

Fermentation

Cell banking

pre-GMP

Downstream processing

Corporate History

Directions

Jobs

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